研究成果

【論文等 (2000年以降)】

“Revisiting biomolecular NMR spectroscopy for promoting small-molecule drug discovery”
H. Hanzawa, T. Shimada, M. Takahashi and H. Takahashi
J. Biomol. NMR, 74, 501-508 (2020)

“Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex”
B. Argunhan, M. Sakakura, N. Afshar, M. Kurihara, K. Ito, T. Maki, S. Kanamaru, Y. Murayama, H. Tsubouchi, M. Takahashi, H. Takahashi, and H. Iwasaki
eLife, 9, e52566 (2020)

“Structural mechanisms underlying activity changes in an AMPA-type glutamate receptor induced by substitutions in its ligand-binding domain”
M. Sakakura, Y. Ohkubo, H. Oshima, S. Re, M. Ito, Y. Sugita, and H.Takahashi
Structure, 27, 1698-1709 (2019)

“Population shift mechanism for partial agonism of AMPA receptor”
H. Oshima, S. Re, M. Sakakura, H. Takahashi, and Y. Sugita
Biophys. J., 116, 57-68 (2019)

“Phosphoinositide binding by the PH domain in ceramide transfer protein (CERT) is inhibited by hyperphosphorylation of an adjacent serine-repeat motif”
T. Sugiki, D. Egawa, K. Kumagai, C. Kojima, T. Fujiwara, K. Takeuchi, I. Shimada, K. Hanada, and H. Takahashi
J. Biol. Chem., 293, 11206-11217 (2018)

“Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system”
R. Suzuki, M. Sakakura, M. Mori, M. Fujii, S. Akashi, and H. Takahashi
J. Biomol. NMR, 71, 213-223 (2018)

“Structural mechanisms for the S-nitrosylation-derived protection of mouse galectin-2 from oxidation-induced inactivation revealed by NMR”
M. Sakakura, M. Tamura, N. Fujii, T. Takeuchi, T. Hatanaka, S. Kishimoto, Y. Arata, and H. Takahashi
FEBS. J., 285, 1129-1145 (2018)

“Allosteric activation of membrane-bound glutamate receptors using coordination chemistry within living cells”
S. Kiyonaka, R. Kubota, Y. Michibata, M. Sakakura, H. Takahashi, T. Numata, R. Inoue, M. Yuzaki, and I. Hamachi
Nat. Chem., 8, 958-967 (2016)

“Suppression of problematic compound oligomerization by cosolubilization of nondetergent sulfobetaines”
Y. Mizukoshi, K. Takeuchi, M. Arutaki, T. Takizawa, H. Hanzawa, H. Takahashi, and I. Shimada
ChemMedChem., 10, 736-741 (2015)

“Dynamic multidrug recognition by multidrug transcriptional repressor LmrR”
K. Takeuchi, Y. Tokunaga, M. Imai, H. Takahashi, and I. Shimada
Scientific Reports, 4, doi:10.1038/srep06922 (2014)

“Allosteric enhancement of MAP kinase p38 alpha's activity and substrate selectivity by docking interactions”
Y. Tokunaga, K. Takeuchi, H. Takahashi, and I. Shimada
Nat. Struct. Mol. Biol., 21, 704-711 (2014)

“Structure-based approach to improve a small molecule inhibitor utilizing a competitive peptide ligand”
K. Ono, K. Takeuchi, H. Ueda, Y. Morita, R. Tanimura, I. Shimada, and H. Takahashi
Angew. Chem. Int. Ed., 53, 2597-2601 (2014)

“Rapid identification of ligand-binding sites by using an assignment-free NMR approach”
Y. Kodama, K. Takeuchi, N. Shimba, K. Ishikawa, E. Suzuki, I. Shimada, and H. Takahashi
J. Med. Chem., 56, 9342-9350 (2013)

“Perdeuteration and methyl-selective ¹H, ¹³C-labeling by using a Kluyveromyces lactis expression system”
M. Miyazawa-Onami, K. Takeuchi, T. Takano, T. Sugiki, I. Shimada, and H. Takahashi
J. Biomol. NMR, 57, 297-304 (2013)

"NMRでリガンド分子の活性部位を探索する"
高橋栄夫
化学, 68, 39-43 (2013)

“Structural Basis for the Golgi Association by the Pleckstrin Homology Domain of the Ceramide Trafficking Protein (CERT)”
T. Sugiki, K. Takeuchi, T. Yamaji, T. Takano, Y. Tokunaga, K. Kumagai, K. Hanada, H. Takahashi, and I. Shimada
J. Biol. Chem., 287, 33706-33718 (2012)

“NMR study of ligand release from asialoglycoprotein receptor under solution conditions in early endosomes”
T. Onizuka, H. Shimizu, Y. Moriwaki, T. Nakano, S. Kanai, I. Shimada, and H. Takahashi
FEBS J., 279, 2645-2656 (2012)

“An accurate pharmacophore mapping method by NMR spectroscopy”
Y. Mizukoshi, A. Abe, T. Takizawa, H. Hanzawa, Y. Fukunishi, I. Shimada, and H. Takahashi
Angew. Chem. Int. Ed., 51, 1362-1365 (2012)

“Isotopic labeling of heterologous proteins in the yeast Pichia pastoris and Kluyveromyces lactis”
T. Sugiki, O. Ichikawa, M. Miyazawa-Onami, I. Shimada, and H. Takahashi
Methods in Molecular Biology (Protein NMR techniques 3rd edit.), 831, 19-36 (2012)

"タンパク質と生体小分子の相互作用２"
高橋栄夫,  嶋田一夫
生体有機化学 （橋本祐一, 村田道雄編著）(東京化学同人) (2012)

“Affinity transfer to a human protein by CDR3 grafting of camelid VHH”
H. Inoue, A. Iihara, H. Takahashi, I. Shimada, I. Ishida, and Y. Maeda
Protein Sci., 20, 1971–1981 (2011)

“Protein–ligand docking guided by ligand pharmacophore-mapping experiment by NMR”
Y. Fukunishi, Y. Mizukoshi, K. Takeuchi, I. Shimada, H. Takahashi, and H. Nakamura
J. Mol. Graph. Mod., 31, 20-27 (2011)

“Rapid identification of protein–protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements”
Y. Kodama, M. L. Reese, N. Shimba, K. Ono, E. Kanamori, V. Dötsch, S. Noguchi, Y. Fukunishi, E. Suzuki, I. Shimada and H. Takahashi
J. Struct. Biol., 174, 434-442 (2011)

“HNCA-TOCSY-CANH experiments with alternate ¹³C-¹²C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment”
K. Takeuchi, M. Gal, H. Takahashi, I. Shimada and G. Wagner
J. Biomol. NMR, 49, 17-26 (2011)

"Structural basis for the Trembler-J phenotype of Charcot-Marie-Tooth disease"
M. Sakakura, A. Hadziselimovic, Z. Wang, K. L. Schey and C. R. Sanders
Structure, 19, 1160-1169 (2011)

“Precise structural determination of weakly binding peptides by utilizing dihedral angle constraints”
Y. Mizukoshi, M. Nagasu, I. Shimada, and H. Takahashi
J. Biomol. NMR, 46, 299-305 (2010)

“Structural Basis for Platelet Anti-aggregation by Angiotensin II type 1-Receptor Antagonist Losartan (DuP-753) via Glycoprotein VI”
K. Ono, H. Ueda, Y. Yoshizawa, D. Akazawa, R. Tanimura, I. Shimada, and H. Takahashi
J. Med. Chem., 53, 2087-2093 (2010)

“Direct determination of the insulin-insulin receptor interface using transferred cross-saturation experiments”
T. Nakamura, H. Takahashi, M. Takahashi, N. Shimba, E. Suzuki, and I. Shimada
J. Med. Chem., 53, 1917-1922 (2010)

“Real-time assay method of lipid extraction activity”
T. Sugiki, H. Takahashi, M. Nagasu, K. Hanada, and I. Shimada
Anal. Biochem., 399, 162-167 (2010)

“Production of isotopically labeled heterologous proteins in non-E.coli prokaryotic and eukaryotic cells”
H. Takahashi and I. Shimada
J. Biomol. NMR, 46, 3-10 (2010)

“Structural Basis of the Interaction between Chemokine Stromal Cell-derived Factor-1/CXCL12 and Its G-protein-coupled Receptor CXCR4”
Y. Kofuku, C. Yoshiura, T. Ueda, H. Terasawa, T. Hirai, S. Tominaga, M. Hirose, Y. Maeda, H. Takahashi, Y. Terashima, K. Matsushima, and I. Shimada
J. Biol. Chem., 284, 35240-35250 (2009)

“Structural Investigation of the Interaction between LolA and LolB Using NMR”
S. Nakada, M. Sakakura, H. Takahashi, S. Okuda, H. Tokuda, and I. Shimada
J. Biol. Chem., 284, 24634-24643 (2009)

“Structural and interaction analysis of glycoprotein VI-binding peptide selected from phage display library”
K. Kato-Takagaki, Y. Mizukoshi, Y. Yoshizawa, D. Akazawa, Y. Torii, K. Ono, R. Tanimura, I. Shimada, and H. Takahashi
J. Biol. Chem., 284, 10720-10727 (2009)

“High-throughput Screening of Optimal Solution Conditions for Structural Biological Studies by Fluorescence Correlation Spectroscopy”
T. Sugiki, C. Yoshiura, Y. Kofuku, T. Ueda, I. Shimada, and H. Takahashi
Protein Sci., 18, 1115-1120 (2009)

"NMRによる蛋白質複合体解析技術"
高橋栄夫
蛋白質核酸酵素増刊号「融合発展する構造生物学とケミカルバイオロジーの最前線」(共立出版), 54, 1634-1640 (2009)

"創薬開発におけるNMR法の活用"
高橋栄夫, 嶋田一夫
実験医学増刊・分子標的薬開発への新たなる挑戦 (羊土社), 27, 771-776 (2009)

“Cross-saturation and transferred cross-saturation experiments”
I. Shimada, T. Ueda, M. Matsumoto, M. Sakakura, M. Osaswa, K. Takeuchi, N. Nishida, and H. Takahashi
Prog. NMR Spectrosc., 54, 123-140 (2008)

“Stable isotope labeling of protein by Kluyveromyces lactis for NMR study”
T. Sugiki, I. Shimada, and H. Takahashi
J. Biomol. NMR, 42, 159-162 (2008)

“Identification and characterization of the slowly exchanging pH-dependenct conformational reaarangement in KcsA”
K. Takeuchi, H. Takahashi, S. Kawano, and I. Shimada
J Biol. Chem., 282, 15179-15186 (2007)

“Pairwise NMR experiments for the determination of protein backbone dihedral angle Φ based on cross-correlated spin relaxation”
H. Takahashi and I. Shimada
J. Biomol. NMR, 37, 179-185 (2007)

“Structural basis of the transition-state stabilization in antibody-catalyzed hydrolysis”
M. Sakakura, H. Takahashi, N. Shimba, I. Fujii, and I. Shimada
J. Mol. Biol., 367, 133-147 (2007)

“Backbone resonance assignments for the periplasmic chaperone LolA from Escherichia coli”
S. Nakada, H. Takahashi, M. Sakakura, M. Kurorno, and I. Shimada
Biomol. NMR Assign., 1, 125-127 (2007)

“Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli”
S. Nakada, M. Sakakura, H. Takahashi, H. Tokuda, and I. Shimada, Biomol. NMR Assign., 1, 121-123 (2007)

"開発候補化合物探索での構造情報（NMR構造解析）の利用"
高橋栄夫
Medical Science Digest, 33, 1065-1068 (2007)

"交換系利用NMRによるリガンド分子の標的分子結合構造解析"
高橋栄夫, 嶋田一夫
蛋白質 核酸 酵素, 52, 959-965 (2007)

“Utilization of methyl proton resonances in cross-saturation measurement for determining the interface of large protein-protein complexes”
H. Takahashi, M. Miyazawa, Y. Ina, Y. Fukunishi, Y. Mizukoshi, H. Nakamura, and I. Shimada
J. Biomol. NMR, 34, 167-177 (2006)

“Rapid preparation of stable isotope labeled peptides that bind to target proteins by phage library system”
Y. Mizukoshi, H. Takahashi, and I. Shimada
J. Biomol. NMR, 34, 23-30 (2006)

“Structural and interaction analysis between GPVI and its peptide ligands selected from phage display library”
K. Kato, H. Takahashi, Y. Mizukoshi, Y. Yoshizawa, T. Sugiki, D. Akazawa, R. Tanimura, and I. Shimada
Peptide Science 2006, 123-124 (2006)

"磁気共鳴分光 III.緩和"
高橋栄夫
分光研究, 55, 205-220 (2006)

“Direct determination of a membrane-peptide interface using the nuclear magnetic resonance cross-saturation method”
T. Nakamura, H. Takahashi, K. Takeuchi, T. Kohno, K. Wakamatsu, and I. Shimada
Biophys. J., 89, 4051-4055 (2005)

“Backbone Resonance Assignment for the Fv Fragment of Catalytic Antibody 6D9 Complexed with a Transition State Analogue”
M. Sakakura, H. Takahashi, H. Terasawa, K. Takeuchi, I. Fujii, and I. Shimada
J. Biomol. NMR, 33, 282 (2005)

“Conformational dynamics of complementary determining region H3 of an anti-dansyl Fv fragment in the presence of its hapten”
M. Nakasako, T. Oka, M. Masumo, H. Takahashi, I. Shimada, Y. Yamaguchi, K. Kato, and Y. Arata
J. Mol. Biol., 351, 627–640 (2005)

“Phage library for molecular recognition analyses by NMR (2): Structure-based functional analyses of selected peptide”
Y. Mizukoshi, M. Nagasu, H. Takahashi, and I. Shimada
Peptide Science 2004, 153-156 (2005)

“Channel-forming membrane permeabilization by an antibacterial protein, sapecin: determination of membrane-buried and oligomerization surfaces by NMR”
K. Takeuchi, H. Takahashi, M. Sugai, H. Iwai, T. Kohno, K. Sekimizu, S. Natori, and I. Shimada
J. Biol. Chem., 279, 4981-4987 (2004)

“Molecular basis of the high-affinity activation of type 1 ryanodine receptors by imperatoxin A”
C. W. Lee, E. H. Lee, K. Takeuchi, H. Takahashi, I. Shimada, K. Sato, S. Y. Shin, D. H. Kim, and J. I. Kim
Biochem. J., 377, 385-394 (2004)

“Phage library for molecular recognition analyses by NMR”
Y. Mizukoshi, H. Takahashi, and I. Shimada
Peptide Science 2003, 23-24 (2004)

“Structural basis of the KcsA K⁺ channel and agitoxin2 pore-blocking toxin interaction by using the transferred cross-saturation method”
K. Takeuchi, M. Yokogawa, T. Matsuda, M. Sugai, S. Kawano, T. Kohno, H. Nakamura, H. Takahashi, and I. Shimada
Structure, 11, 1381-1392 (2003)

“Novel collagen-binding mode of the VWA domain determined by a transferred cross-saturation experiment”
N. Nishida, H. Sumikawa, M. Sakakura, N. Shimba, H. Takahashi, H. Terasawa, E. Suzuki, and I. Shimada
Nat. Struct. Biol., 10, 53-58 (2003)

“Backbone ¹H, ¹³C, ¹⁵N resonance assignments of the von Willebrand factor A3 domain”
N. Nishida, M. Miyazawa, H. Sumikawa, M. Sakakura, N. Shimba, H. Takahashi, H. Terasawa, E. Suzuki, and I. Shimada
J. Biomol. NMR, 24, 357-358 (2002)

“Solution structure of ω-grammotoxin SIA, a gating modifier of P/Q and N-type Ca²⁺ channel”
K. Takeuchi, E. J. Park, C. W. Lee, J. I. Kim, H. Takahashi, K. J. Swartz, and I. Shimada
J. Mol. Biol., 321, 517-526 (2002)

“Determination of the interface of a large protein complex by transferred cross-saturation measurements”
T. Nakanishi, M. Miyazawa, M. Sakakura, H. Terasawa, H. Takahashi, and I. Shimada
J. Mol. Biol., 318, 245-249 (2002)

“Identification and Characterization of an Antibacterial Peptide of the 26-kDa Protease of Sarcophaga peregrina with Antibacterial Activity”
Y. Tsuji, T. Aoyama, K. Takeuchi, K. Homma, H. Takahashi, Y. Nakajima, I. Shimada, and S. Natori
J. Biochemistry, 130, 313-318 (2001)

“Solution Structure of Micelle-bound H5 Peptide (427-452): A Primary Structure Corresponding to the Pore-Forming Region of the Voltage Dependent Pottasium Channel”
K. Shindo, H. Takahashi, K. Shinozaki, K. Kami, K. Anzai, S. Lee, H. Aoyagi, Y. Kirino, and I. Shimada
Biochem. Biophys. Acta, 1545, 153-159 (2001)

“Backbone ¹H, ¹³C, ¹⁵N resonance assignments of the anti-dansyl antibody Fv fragment”
K. Shindo, K. Masuda, H. Takahashi, Y. Arata, and I. Shimada
J. Biomol. NMR, 17, 357-358 (2000)

“A novel NMR method for determining the interfaces of large protein-protein complexes”
H. Takahashi, T. Nakanishi, K. Kami, Y. Arata, and I. Shimada
Nat. Struct. Biol., 7, 220-223 (2000)

“Solution structure of hanatoxin1, a gating modifier of voltage-dependent K⁺ channels: common surface features of gating modifier toxins”
H. Takahashi, J. I. Kim, H. J. Min, K. Sato, K. J. Swartz, and I. Shimada
J. Mol. Biol., 297, 771-780 (2000)

【特許】

「結合部位同定装置、結合部位同定方法および結合部位同定プログラム」
小玉優哉、竹内恒、高橋栄夫、嶋田一夫，
特許第6344768号

「ファージディスプレイ法を用いた分子間相互作用解析方法」
水越弓子、高橋栄夫、嶋田一夫
特許第4184907号

「蛋白質複合体解析法」
高橋栄夫、嶋田一夫、中村壮史
特許3947775号