横浜市立大学 生命医科学研究科

    構造エピゲノム科学研究室

         助教

         小沼　剛　Ph.D.

          Lab : B305

          E-mail : konumax[at]yokohama-cu.ac.jp

                    [at]を@に変えて下さい

【研究内容】

　核磁気共鳴（NMR）やX線を利用し、蛋白質や核酸などの生体分子の相互作用解析や立体構造解析を行い生命活動の基礎を研究する。また、がんなど重篤な疾病に関わる蛋白質について、その立体構造に基づいた抗がん剤の開発を行う。

【主要論文】

1.     Konuma T, Zhou MM

        “Distinct Histone H3 lysine 27 modifications dictate different outcomes of gene transcription”

        J. Mol. Biol. In press

2.     #Liu N, #Konuma T, Sharma R, Wang D, Zhao N, Cao L, Ju Y, Liu D, Wang S, Bosch A, Sun Y, Zhang S, Ji D, Nagatoishi S, Suzuki N, Kikuchi M, Wakamori M, Zhao CC, Ren C, Zhou J T, Xu Y, Meslamani J, Fu S, Umehara T, Tsumoto K, Akashi S, Zeng L, Roeder G R, Walsh J M, Zhang Q, Zhou MM

“Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin”

Mol. Cell, 83, 2206-2221 (2023) Seleced as Featured Article (#Co-first author)

3.     Konuma T, Kurita J, Ikegami T

“CPMG pulse sequence for relaxation dispersion that cancels artifacts independently of spin states”

J. Mag. Res., 352, 107489 (2023)

4.     Azegami N, Taguchi R, Suzuki N, Sakata Y, *Konuma T, *Akashi S

“Native mass spectrometry of BRD4 bromodomains linked to a long disordered region”

Mass Spectrom. (Tokyo), 11, A0110 (2022) (*Co-corresponding author)

5.     Hata K, Kobayashi N, Sugimura K, Qin W, Haxholli D, Chiba Y, Yoshimi S, Hayashi G, Onoda H, Ikegami T, Mulholland BC, Nishiyama A, Nakanishi M, Leonhardt H, *Konuma T, *Arita K

“Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger”

Nucleic. Acids. Res., 50, 12527-12542 (2022) (*Co-corresponding author)

6.     Sakamoto W, Azegami N, Konuma T, Akashi S

        “Single cell native mass spectrometry of human erythrocytes”

        Anal. Chem., 93, 6583-6588 (2021)

7.     #Imagawa E, #Konuma T, #Cork EE, Diaz GA, Oishi K

        “A novel missense variant in RBM10 can cause a mild form of TARP syndrome with development delay, growth restriction and dysmorphic features”

        Clin. Genet., 98, 606-612 (2020) (#Co-first author)

8.     Schuller JM, Birrell JA, Tanaka H, Konuma T, Wulfhorst H, Cox N, Schuller SK, Thiemann J, Lubitz W, Sétif P, Ikegami T, Engel BD, Kurisu G, Nowaczyk MM

“Structural adaptations of photosynthetic complex I enable ferredoxin-dependent electron transfer”

Science, 363, 257-260 (2019)

9.     Konuma T, Nagadoi A, Kurita J, Ikegami T

“Analysis of artifacts caused by pulse imperfections in CPMG pulse trains in NMR relaxation dispersion experiments”

Magnetochemistry, 4, 33-50 (2018)

10.   Konuma T, Yu D, Zhao CC, Ju Y, Sharma R, Ren C, Zhang Q, Zhou MM, Zeng L

“Structural mechanism of the oxygenase JMD6 recognition by the extraterminal (ET) domain of BRD4”

Sci. Rep., 7, 16272-16281 (2017)

Researchmap

【研究助成】